LOCUS       UGT1A4-pGEc2            6699 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6177..6306)
                     /note="3'-ITR"
     misc_feature    complement(5875..6089)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5327..5868
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4506..4528
                     /note="attB2"
     misc_feature    5306..5311
                     /note="BstB1 site"
     misc_feature    4529..5224
                     /note="Super GFP"
     misc_feature    5225..5275
                     /note="AviTag"
     misc_feature    5276..5299
                     /note="His Tag"
     misc_feature    3011..4483
                     /note="OPTIMIZED UGT1A4 coding region"
     PCR_frame       3011..4505
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    4484..4505
                     /note="TEV site"
     vector          join(4514..6699,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4513
                     /source="UGT1A4 pDONR"
                     /type="Entry Clone"
     source          1..6699
                     /dnas_title="UGT1A4-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCCAGAG GACTGCAGGT CCCACTGCCT AGACTGGCTA CAGGACTGCT
     3061 GCTGCTCCTG TCTGTGCAGC CTTGGGCCGA ATCTGGAAAA GTGCTGGTGG TGCCTACAGA
     3121 CGGCAGCCCT TGGCTGTCTA TGAGAGAGGC CCTGAGAGAA CTGCACGCTA GAGGACATCA
     3181 GGCCGTGGTG CTGACCCCCG AAGTGAACAT GCACATCAAA GAGGAAAAGT TCTTCACCCT
     3241 GACCGCCTAC GCCGTGCCCT GGACCCAGAA AGAATTCGAT AGAGTGACCC TGGGCTACAC
     3301 CCAGGGCTTT TTCGAGACAG AGCATCTGCT GAAGCGGTAC AGCCGCAGCA TGGCCATCAT
     3361 GAACAATGTG TCTCTGGCCC TGCACAGGTG CTGCGTGGAA CTGCTGCACA ACGAAGCCCT
     3421 GATCAGACAC CTGAACGCCA CCAGCTTCGA TGTGGTCCTG ACCGACCCTG TGAATCTGTG
     3481 TGGCGCCGTG CTGGCCAAGT ATCTGTCTAT CCCTGCCGTG TTCTTCTGGC GGTACATCCC
     3541 CTGCGATCTG GACTTCAAGG GCACCCAGTG TCCCAACCCT AGCAGCTACA TTCCCAAGCT
     3601 GCTGACCACC AACAGCGACC ACATGACCTT TCTGCAGCGC GTGAAGAATA TGCTGTACCC
     3661 CCTGGCTCTG AGCTACATCT GCCACACCTT TAGCGCCCCT TATGCCAGCC TGGCCTCTGA
     3721 ACTCTTTCAG CGGGAAGTGT CCGTGGTGGA TCTGGTGTCT TATGCCTCCG TGTGGCTGTT
     3781 CCGGGGCGAC TTCGTGATGG ACTACCCCAG ACCCATCATG CCCAACATGG TGTTCATCGG
     3841 CGGCATCAAC TGCGCCAATG GAAAGCCCCT GAGCCAGGAA TTCGAGGCCT ACATCAATGC
     3901 CAGCGGCGAG CACGGCATCG TGGTGTTTTC TCTGGGATCC ATGGTGTCCG AGATCCCCGA
     3961 GAAGAAAGCC ATGGCAATCG CTGACGCCCT GGGCAAGATT CCTCAGACAG TGCTGTGGCG
     4021 CTACACCGGC ACAAGACCTA GCAACCTGGC CAACAACACC ATCCTGGTCA AGTGGCTGCC
     4081 CCAGAATGAT CTGCTGGGCC ACCCTATGAC CAGAGCCTTT ATCACACACG CCGGCTCTCA
     4141 CGGCGTGTAC GAGTCTATCT GTAATGGCGT GCCCATGGTC ATGATGCCCC TGTTCGGCGA
     4201 CCAGATGGAC AACGCCAAGC GGATGGAAAC AAAGGGCGCT GGCGTGACAC TGAACGTGCT
     4261 GGAAATGACC AGCGAGGACC TGGAAAATGC CCTGAAGGCC GTGATCAACG ACAAGAGCTA
     4321 CAAAGAAAAC ATCATGCGGC TGAGCAGCCT GCACAAGGAC AGACCTGTGG AACCTCTGGA
     4381 CCTGGCCGTG TTTTGGGTGG AATTTGTGAT GCGGCACAAA GGCGCCCCTC ATCTGAGGCC
     4441 AGCTGCTCAC GATCTGACCT GGTATCAGTA CCACAGCCTG GACgaaaact tgtactttca
     4501 aggctCCAGC TTTCTTGTAC AAAGTGGTAT GAGCAAGGGC GAGGAACTGT TCACCGGCGT
     4561 GGTGCCCATC CTGGTGGAAC TGGATGGCGA CGTGAACGGC CACAAGTTCT CTGTGCGGGG
     4621 AGAGGGCGAA GGCGACGCCA CAAATGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG
     4681 CAAGCTGCCC GTGCCTTGGC CTACCCTCGT GACCACACTG ACCTACGGCG TGCAGTGCTT
     4741 CAGCAGATAC CCCGACCATA TGAAGCGGCA CGACTTCTTC AAGAGCGCCA TGCCCGAGGG
     4801 CTACGTGCAG GAACGGACCA TCAGCTTCAA GGACGACGGC ACCTACAAGA CCAGAGCCGA
     4861 AGTGAAGTTC GAGGGCGACA CCCTCGTGAA CCGGATCGAG CTGAAGGGCA TCGATTTCAA
     4921 AGAGGACGGC AACATCCTGG GCCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTGTA
     4981 CATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC GGCACAACGT
     5041 GGAAGATGGC AGCGTGCAGC TGGCCGACCA CTACCAGCAG AACACCCCCA TCGGAGATGG
     5101 CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG AGCGTGCTGA GCAAGGACCC
     5161 CAACGAGAAG CGGGACCACA TGGTGCTGCT GGAATTCGTG ACCGCCGCTG GCATCACCCA
     5221 CGGCATGAGC GGCCTGAACG ACATCTTCGA GGCCCAGAAA ATCGAGTGGC ACGAGCACCA
     5281 CCATCATCAC CATCACCACT GATGATTCGA AAAATAATAA GGATCCAATC AACCTCTGGA
     5341 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG
     5401 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT
     5461 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG
     5521 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC
     5581 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA
     5641 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA
     5701 TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC
     5761 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT
     5821 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG
     5881 ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC
     5941 CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT
     6001 CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT
     6061 TGGGAAGACA ATAGCAGGCA TGCTGGGGAC TCGAGTTAAG GGCGAATTGC CGATTAGGAT
     6121 CTTCCTAGAG CATGGCTACG TAGATAAGTA GCATGGCGGG TTAATCATTA ACTACAAGGA
     6181 ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG
     6241 GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC
     6301 GCGCAGCCTT AATTAAGAGC TCATGGCGCG CCTAGGCCTT GACGGCCTTC CGCCAATTCG
     6361 CCCTATAGTG AGTCGTATTA CGTCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG
     6421 GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG
     6481 GCGTAATAGC GAAGAGGCCC GCACCGAAAC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG
     6541 GCGAATGGGA GCGCCCTGTA GCGGCCACTC AACCCTATCT CGGTCTATTC TTTTGATTTA
     6601 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
     6661 AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAG
//

